Journal: Molecular Cancer

Article Title: NAC1 promotes stemness and regulates myeloid-derived cell status in triple-negative breast cancer

doi: 10.1186/s12943-024-02102-y

Figure Lengend Snippet: Comparison of tumor growth of NAC1-expressing and NAC1-deficient TNBC cells in nude or NSG mice ( n = 3, Tn = 2). A Illustration of tumor orthotopic inoculation in nude and NSG mice models. Mice were orthotopically injected with MDA-MB-231 cells (1 × 10 6 cells/injection, n = 4, Tn = 2 on the 4th mammary fat pad) with or without depletion of NAC1, and tumor growth was monitored every five days until mice showed adverse clinical symptoms due to increased tumor burden. B , C Tumor growth in nu/nu mice. D , E Tumor growth in NSG mice. To evaluate the infiltration of MDSCs and NK cells into the tumor microenvironment, we performed an immunofluorescence assay using Ly6G for MDSCs and NK1.1 and CD16 antibodies for NK cells. F Tumor infiltration of MDSCs in the tumor-bearing nude or NSG mice. G Tumor infiltration of NK cells in tumor-bearing nude mice, as shown by staining with NK1.1 and CD16 antibodies. Red arrows indicate the inactive NK cells

Article Snippet: To deplete MDSCs in nu/nu mice, animals were administrated intraperitoneally with control IgG (mouse IgG2A isotype control, Clone: C1.18.4, Biocell) or anti-Ly6G antibody (anti-mouse Ly6G, Clone: 1A8, Biocell) at a dose of 4 mg/kg/mouse every four days [ ] for a period of 20 days.

Techniques: Comparison, Expressing, Injection, Immunofluorescence, Staining

Journal: Molecular Cancer

Article Title: NAC1 promotes stemness and regulates myeloid-derived cell status in triple-negative breast cancer

doi: 10.1186/s12943-024-02102-y

Figure Lengend Snippet: Effects of NK cells on growth of NAC1-expressing and NAC1-deficient TNBC cells in nude mice. A Illustration of NK cell depletion approach. Mice were orthotopically inoculated with the luciferase plasmid transfected-MDA-MB-231 cells with or without depletion of NAC1 (1 × 10 6 cells/inoculation, n = 4, Tn = 4). On day four following tumor inoculation, the mice were given NK1.1 antibody or control IgG (4 mg/kg) intraperitoneally five times with a four day-interval to deplete NK cells. Tumor cell proliferation and tumor growth were monitored using the Lago optical imaging system. B , C Luminescence intensity of tumor cells grown in mice with or without NK cell depletion ( B ) and quantification of the luminescence intensity (photon emission) ( C ). D Tumor volume (mm 3 ) in mice with or without NK cell depletion. E Tumor formations in the mice treated with NK1.1 antibody or IgG. F Tumor weights in mice with or without depletion of NK cells. G Illustration of MDSCs depletion approach. Mice were orthotopically inoculated with the luciferase plasmid transfected-MDA-MB-231 cells (1 × 10 6 cells/inoculation, n = 2, Tn = 4) with or without depletion of NAC1. On day four following tumor inoculation, the mice were given Ly6G antibody or control IgG (4 mg/kg) intraperitoneally five times with a four day-interval to deplete MDSCs. Tumor cell proliferation and tumor growth were monitored using the Lago optical imaging system. H , I Luminescence intensity of tumor cells grown in mice with or without depletion of MDSCs (H) and quantification of the luminescence intensity (photon emission) obtained on the last day of antibody depletion treatment. J Tumor volume (mm 3 ) in mice with or without depletion of MDSCs. K Tumor formations in the mice treated with Ly6G antibody or IgG. L Tumor weights in mice with or without depletion of MDSCs

Article Snippet: To deplete MDSCs in nu/nu mice, animals were administrated intraperitoneally with control IgG (mouse IgG2A isotype control, Clone: C1.18.4, Biocell) or anti-Ly6G antibody (anti-mouse Ly6G, Clone: 1A8, Biocell) at a dose of 4 mg/kg/mouse every four days [ ] for a period of 20 days.

Techniques: Expressing, Luciferase, Plasmid Preparation, Transfection, Control, Optical Imaging

Journal: iScience

Article Title: β-cell function is regulated by metabolic and epigenetic programming of islet-associated macrophages, involving Axl, Mertk, and TGFβ receptor signaling

doi: 10.1016/j.isci.2023.106477

Figure Lengend Snippet: Key resources table

Article Snippet: Ly-6G APC/Cy7 clone 1A8 , BD Diagnostics , Cat#562700; RRID AB_2737730.

Techniques: Recombinant, Liposomes, Control, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Software